Characterization of Miniature Probes for Cryosurgery, Thermal Ablation, and Irreversible Electroporation on Small Animals

Abstract Focal therapies, including cryosurgery, thermal ablation, and irreversible electroporation (IRE) are widely used in the treatment of cancer other diseases, aimed at exposing cells to extreme physical conditions leading cell death. ablation outcomes extensively studied small animal preclinical models, such as mice; however, robust engineering characterization these treatments is largely lacking, due a large part lack control applying clinical devices (cm scale) mouse tumors (mm scale). To address this issue, miniaturized probes designed built for controlled application IRE their performance evaluated using ex vivo porcine liver tissue an MC‐38 tumor model. These experimental results compared simulated heat transfer (cryosurgery ablation) electrical models. Agreements quantities between measurement modeling shown all focal therapy cases. The outcome further correlated with growth delay. improves understanding energy dose create damage, therefore enables better understand studies predict optimize future procedures.